Development projects with their positive effects always have potential direct and indirect negative effects on the human and natural environment, and if the negative effects are not thought of in advance and are not strengthened in terms of positive effects, they will have adverse results and consequences. The purpose of the research is to evaluate the biological environment of the irrigation and drainage network of Khodaafrin dam in East Azarbaijan province and Ardabil in Khodaafrin and Parsabad cities. In order to investigate environmental parameters, three physical-pollution, ecological-biological and economic-social and cultural environments were used. Then, using the Icold matrix method, the effect of each of the micro-activities of the project on the environmental factors of the study area was measured in the exploitation phase, separately from the three mentioned environments. The findings showed that the implementation of the plan is preferable to its non-implementation in such a way that the total consequences of the implementation of the plan on the surrounding environment are positive 274 points. Among the consequences of the implementation of the socio-economic and cultural environment plan, it has the most positive effects of 186 points, physical environment and pollution with 46 positive points, and ecological and biological environment with 42 positive points. According to the findings, the implementation of the irrigation and drainage network plan of the main KhodaAfarin canal is justified by complying with the standards. In order to reduce the negative effects on the three environments, it is suggested to consider continuous and stable monitoring and compliance with the correct principles of irrigation during the operation of the project.